Development of Acrylic Acid Grafted Polycaprolactone (PCL)/Biphasic Calcium Phosphate (BCP) Nanofibers for Bone Tissue Engineering Using Gamma-Irradiation

摘要

Polycaprolactone (PCL)? biphasic calcium phosphate (BCP)? ????? ? ? ?? ?? ??? ?? ???? ??? ???? ??. ???,PCL? ??? ????? ?? ??? ?? ? ??? ????? ??? ?? ???? ?? ? ????? ???? ???? ? ??? ?? ???? ??? PLC/BCP ????? ?????.????? ????? ?? ??????, ???? ???? acrylic acid (AAc)? ?????. SEM? ?? ?? ?? ??? ?????, AAc? ??? ????? ??? MG63? ?? ???? ??? ??? ?? ?????. ???? ?????? ??? 1.239 +/- 0.226 nmole/mu g/min?? ???? ?? ????(0.590 +/- 0.286 nmole/mu g/min)?? ?????.??? AAc? ??? PCL/BCP ????? ??? ??? ??? ? ?? ??? ????. Polycaprolactone (PCL) and biphasic calcium phosphate (BCP) have been considered as useful materials for orthopedic devices and osseous implants because of their biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces reducing initial cell adhesion or proliferation. To overcome the limitation, we fabricated surface-modified PCL/BCP nanofibers using gamma-irradiation for bone tissue engineering. PCL/BCP nanofibers were prepared by electrospinning and then we supplemented hydrophilicity by introducing acrylic acid (AAc) through gamma-irradiation. We confirmed the surface of nanofibers by SEM, and then the initial viability of MG63 was significantly increased on the AAc grafted nanofibers, and alkaline phosphatase activity(1.239 +/- 0.226 nmole/mu g/min) improved on the modified nanofibers than that on the non-modified nanofibers(0.590 +/- 0.286 nmole/mu g/min). Therefore, AAc-grafted nanofibers may be a good tool for bone tissue engineering applications.